Separation Techniques & Formulation Reports

Biography

Biography: Noha Fadl

Abstract

This  study  aims  to  analysis  fractions  (petroleum ether, chloroform,  methanol  and  aqueous)  fermented wood Nikhra  of  Combretaceae  spp  (Combretum  hartmannianum  and  Terminalia  laxiflora)  analysis  it  by  using chromatographic  and  spectroscopic  analysis.  Petroleum  ether  Nikhra  fraction  analysis  with  TLC  and  spray TLC  with  vanillin H₂SO₄  (pink)  (B1, B2), Rf  values (0.84, 0.81),  were expected to be phenolic,  with  vanillin HCL  (red) compounds spots (B2)  with Rf values (0.81)  was expected to be catechin and with  vanillin H3PO4, blue-violet zones  compounds spots (A1, A2, A3), (B1, B2, B3, B4),  with Rf values  (0.88, 0.78, 0.67),  (0.84, 0.81, 0.67, 0.59),  respectively were expected to be lignans.  Petroleum ether  fermented wood"Nikhra"  fraction was  divided  into  two  types  of  compounds  classes  aromatic  and  non-aromatic  and  hence  compounds  were classified  to phenolics and terpenoids compounds  by  GC/MS. Fragrant aromatics or terpenoids were targeted in this part of study.  GC-MS analysis gave a spectrum  of fragrance  aromatic compounds (phenolics) in the petroleum ether  Nikhrafractions of  T. laxiflora,  was Lup-20(29)-en-3-ol, acetate, (3β)  and  Tetracosamethylcyclododecasiloxane,  main  terponoids  compounds  were  eicosamethylcyclodecasiloxane.  Main  fragrance aromatics  compound  in  the  petroleum  ether  Nikhra  fraction  of  C.  hartmannianum  was  2-tert-Butyl-5-(hydroxtmethyl)-4-formylfuran, and main terponoids compound was Tetracosamethylcyclododecasiloxane.